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Single-point ligand binding experiments were conducted at a [3H]NT concentration of 2 nM, and the amount of specifically bound [3H]NT was then corrected for fractional occupancy using the respective Kd value [12].
Whole-cell binding experiments were conducted as described previously [ 17].
Single-point ligand binding experiments and saturation binding experiments were conducted as described previously.
Nuclear extract preparation and NF-κB DNA binding experiments were conducted with kits purchased from Active Motif.
The calcium binding experiments were conducted at 37 °C, and lipid binding was conducted at 30 °C.
Further, Annexin-V binding experiments were conducted in both cell lines to determine the probable mechanism of cell death.
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Equilibrium binding experiments are conducted to evaluate the binding characteristics of MIPs and templates (BPA and 2,4-D).
Biomolecular binding experiments are conducted on an external gold electrode situated within a custom-designed flow cell and electrically connected to the micromachined electrometer.
In order to assay their influence, if any, on nucleotide binding, fluorescent experiments were conducted using mGDP and mGTP.
To control for nonspecific Ab binding, isotype control experiments were conducted and resulted in nonsignificant background stains.
The data were fit to a first-order binding equation in Origin: 2 Experiments were conducted at 4 °C on a Beckman Optima XL-Analytical ultracentrifuge using an An-60 Ti rotor.
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