Sentence examples for binding experiments using the from inspiring English sources
To test this, we performed competition binding experiments using the extracellular domains of recombinant IL-1RI and IL-1RII.
Figure 3 Competition binding experiments using the NPY Y 1 receptor-expressing SK-N-MC cell line.
Moreover, although residual electron density was observed for t1G and t1C nucleotides, binding experiments using the same target RNAs show that these interactions do not contribute substantially to target affinity.
Briefly, the density of expressed β-ARs on UROtsa cells was determined by saturation binding experiments using the nonselective β-AR antagonist I-CYP as the radiolabel (NEN Life Sciences, Boston, MA).
The whole cell binding experiments used the same method but without sonication and membrane preparation.
Then, an in vitro binding experiment using the recombinant proteins of glutathione-S-transferase (GST -fused Hsp60 and (His)6-taGST -fusedore subunits was assessed by a GST pull-down assay.
To further assess whether the p52 Bcl3 complex senses A/T-and G/C-centric κB sites differently, we carried out in vitro DNA-binding experiments using the electrophoretic mobility shift assay (EMSA).
Binding experiments, using CHO cells expressing the human SP-specific NK1 receptor, demonstrated the functionality of all peptidylcyclodextrin derivatives, which exhibited IC50 values in a 10−9 10−7 M range.
To measure the affinity of native CLOCK BMAL1 protein-DNA interactions, we performed saturation binding experiments using EMSA to determine the apparent kd and Bmax values for the different complexes and E-box sites.
Hence, we performed binding experiments using cold E2, MIBE, the selective GPER ligand G-1 and OHT, which has been largely reported to act as a GPER agonist [ 7].
To determine whether this interaction occurs at the surface of MDA-MB-231 cells (a breast cancer cell line that has high endogenous uPA expression [ 27]), binding experiments using antibodies directed against the A- or B-chain of uPA were performed under conditions that predominantly exclude proteolytic activation of plg by uPA.
