Sentence examples for binding experiments using a from inspiring English sources

Moreover, the binding experiments using a monomeric CRP-specific DNA aptamer revealed that pentameric CRP underwent structural transition into the monomers following the adsorption onto the surfaces via multivalent contacts in a pH-dependent manner.

Phe120ephrinB2 was confirmed as being crucial to the interaction by site-directed mutagenesis and binding experiments using a F120AephrinB2 mutant which was found to be incapable of binding NiV-G [ 44].

Non-human primate cross-reactivity of CDP7657 was measured in saturation binding experiments using a broad-range antibody titration on peripheral blood lymphocytes isolated from humans, Rhesus monkeys and Cynomolgus monkeys and activated in vitro to express CD40L.

Although our binding experiments using a single type of (CR x species precluded being able to simultaneously bind different fragments of cluster II to RAP, it is clear from Table 1 that RAP could tightly engage CR3 CR8, with D1 binding to CR345 (Kd of 23 nM) and D3 binding to CR678 (Kd of 26 nM).

In this study, a homologous series of unsubstituted and 4-tert-butyl-substituted bispyridinium compounds with different alkane linker lengths was investigated in competition binding experiments using [3H]epibatidine as a reporter ligand.

To determine whether this interaction occurs at the surface of MDA-MB-231 cells (a breast cancer cell line that has high endogenous uPA expression [ 27]), binding experiments using antibodies directed against the A- or B-chain of uPA were performed under conditions that predominantly exclude proteolytic activation of plg by uPA.

Chromatogram binding experiments using 125I-labeled subunits gave a specific binding of the CssB subunit to sulfatide, the major acid glycosphingolipid of piglet intestine [16] (Fig. 6D, lane 8), in the same manner as the CS6 protein (Fig. 6B, lane 8).

Through a series of binding experiments using Surface Plasmon Resonance, they provide information regarding the reaction kinetics with the lysine residue.

In receptor binding experiments using cloned human receptors SLV329 behaved as a potent (pKi 9.2) and selective A1R ligand.

Immunoprecipitation and affinity binding experiments using human HEK293 cells cotransfected with cDNAs for FLAG-tagged DICER, a member of the ribonuclease III family, and the His-tagged members of the Argonaute family suggested that the proteins from members of both subfamilies are associated with DICER.

The WNK4 D564A mutation would similarly disrupt this interaction and indeed our independent binding experiments using either KLHL3 R528H or WNK4 D564A confirm the importance of this salt bridge for a high-affinity interaction.