One of the first approaches allowing homogeneous binding experiments was based on the optical separation of bound and free fluorescent molecules, the so-called laser-scanning imaging (LSI).
Experiments were based on duplicate biological replicates.
In principle, since this experiment is based on affinity selection, stronger binding to bait proteins should correspond to a higher probability of detection.
The experiment was based on testing of antibody binding to HER2 proteins that have been modified by deletion of different subdomains of its ECD.
Equilibrium binding experiments are conducted to evaluate the binding characteristics of MIPs and templates (BPA and 2,4-D).
RNA binding experiments was measured as previously described [21], [22].
Ribosome binding experiments were performed as previously described [24].
All binding experiments were performed twice.
Single-point ligand binding experiments and saturation binding experiments were conducted as described previously.
Binding experiments were carried out at 4°C for 1 hr.
