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Competitive binding experiments indicate that these three compounds bind significantly stronger to warfarin compared to diazepam binding site.
DNA binding experiments indicate that both mutants retain the ability to bind AP site-containing DNA with similar affinity as wild-type Tdp1 (Fig. 5A).
LNA binding experiments indicate that unwinding of this helix induces a major structural rearrangement throughout the frameshift domain.
Protein binding experiment indicated that 1 3 could bind to bovine serum albumin (BSA) with moderate bonding via the static quenching mechanism.
Radioligand-binding experiments indicated that mtCXCL12 had an affinity for CXCR4 that was identical to the wild-type sequence (Fig. 1 A ).
In vitro nerve agent binding and inhibition experiments indicated that the fusion protein in the milk of transgenic mice had similar inhibition characteristics compared to human plasma BChE against the nerve agents tested.
DBDs were used to determine binding profiles, as initial experiments indicated that significant differences were not observed between profiles obtained using full-length proteins or DBDs for GABPα or ETS1 (Supplementary Figure S9A).
These experiments indicated that PrC binding leads to a significant decrease in the surface hydrophobicity of the protein (Text S1).
(20) Gel filtration and NMR experiments indicated that calcium-binding to S100B leads to an ∼300-fold increase in affinity for IC3-C58 compared to the apo-state.
In addition, our HSQC titration experiments indicated that α and β binding interfaces on skelemin surface do not overlap, but are located on the opposite sides of the domain 5, rendering the possibility of competitive binding very unlikely.
Single-point mutation experiments indicated that naproxen targets the RNA binding groove of NP and prevents its interaction with virus RNA.
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