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The binding experiments did not reveal any significant difference in the inhibitory activity of peptide 15K in comparison with the scrambled analog (Figure 5D).
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However, the peptide binding experiments do not suggest that Pellino3a shows any significant preference for this motif.
To ensure the tetramer-binding experiments did not underestimate the number of T cells that cross-react with both peptide variants, we analyzed intracellular IFNγ production by splenocytes from vaccinated mice stimulated with either the F1A5 or the WMF peptides (Fig. 2c, d).
Punctate labeling is diminished in images of tissue near the tracks of probes perfused with aCSF, which also exhibit diffuse TH labeling: control experiments did not reveal nonspecific binding, which indicates that the diffuse labeling is specific binding.
Thus, similar to the counter-selection cycles, the negative selection experiments did not reduce RNA binding to Aβ40 fibrils.
ITC experiments did not detect Cyr61 binding to the SMTB 1 44 domain in solution, and competitive ELISA showed that monomeric VTNC reacts weakly if at all with Cyr61; however, modified monomeric VTNC displays higher reactivity, suggesting that cryptic epitopes for Cyr61 were exposed by heat treatment as reported for VTNC interaction with specific antibodies [14], [29].
Eliminating DNA in these co-IP experiments did not alter the binding interactions we observed between TAF7L and PPARγ or TBP (data now shown).
Notably, in our hands ChIP-qPCR experiments did not lend support for the binding of CEBPb.
These experiments did not, therefore, provide a compelling argument for simultaneous binding of histones and Pho4 to N-2 sequences.
I also thought that in vivo experiments did not convincingly establish a role for the Rab6 nucleotide switch on regulating KIF1C motor/microtubule binding function in vivo.
Those experiments did not last.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com