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Annexin V binding experiments demonstrate that apoptosis was induced by CLE in both the breast carcinoma cell lines.
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In vitro and in vivo binding experiments demonstrated that Pin1 binds to N1-ICD and this interaction was direct (supplementary Fig S5B and C).
This notion is supported by recent in vitro binding experiments demonstrating that a FUS fragment (residues 278 385) containing the RRM domain binds DNA with micromolar binding affinity (Liu et al., 2013).
Binding experiments demonstrated that optimized hybrid MIP foams (MIPFs-M) possessed enhanced adsorption capacity, fast kinetics and excellent recognition ability towards λ-cyhalothrin (LC).
Results obtained from the batch binding experiments demonstrated that all of the TIPs have sites that have selective binding ability for theophylline, but not to another structurally similar molecule, caffeine.
Binding experiments demonstrated that BCO allele-specific sequences impaired tag-antitag interaction to a greater extent than predicted by a standard algorithm for secondary structure.
These in vitro binding experiments demonstrated that EEA1 had an intrinsic affinity to Ube2A with Kd of ~9.7 ± 0.54 μM.
Binding experiments demonstrated a specific interaction between SENP1 and Elk-1.
Unlike the computational study described above, in which the hydroxamate moieties of ligands are predicted to be equally deprotonated upon their binding to HDAC8, our ITC experiments demonstrate that the extents of deprotonation of the zinc-binding groups of the ligands (upon binding to the enzyme) are remarkably different from one another (Table 2).
Control experiments demonstrate that the binding of 11A8 to infected cells is specifically blocked by preincubation of antibody with homologous MCP (Figure 7) and that 11A8 does not detect gp120 passively bound to CD4 on cells (Table 6).
Nevertheless, site-directed mutagenesis experiments demonstrate that metal binding behavior can be easily reintroduced into the TbARG protein, so the α/β scaffold appears to be readily evolved by nature or by design.
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