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Molecular modeling, binding experiments and functional assays were performed in order to evaluate the biological action exerted by MIBE through ERα and GPER in MCF7 and SkBr3 breast cancer cells.
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The target compounds, which were assayed in binding experiments and electrophysiological, functional and Erk1/2 activation tests, essentially combined the pharmacological profiles of their individual receptor ligands.
Binding experiments and Scatchard analysis indicated the presence of specific binding sites in the imprinted polymers.
Kathryn Sarachan and Gregory Van Duyne performed binding experiments and analysed data.
Single-point ligand binding experiments and saturation binding experiments were conducted as described previously.
Frances-Rose Frances-Rose Schumachere fluorescent binding experformed and analysis.
LT carried out the binding experiments and statistical analysis.
Thus, the above-mentioned approach is suitable for kinetic binding experiments and visual confirmation of receptor localization, allowing not only localized saturation binding experiments but also internalization experiments.
The data suggest that 5-HTBP is a good structural model for examining ligand binding interactions, and functional experiments on the 5-HT3 receptors yield additional information on binding modes that cannot readily be ascertained from structural information.
We separated an oxidized IgG1 monoclonal antibody based on the oxidation state of methionine 252 and analyzed fractionated material in receptor binding experiments as well as in functional (cell-based) assays.
Based on binding experiments, αβ integrins and their subtypes have been identified as major functional adhesion receptors on tumor cells.
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