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STRING database analysis shows its interaction with GAP50 acid phosphatase on the basis of binding evidences.
We analyzed gene expression profiles along with these binding evidences to discriminate functional from NFB of a TF.
ChIP-chip profiles generated under a particular experimental condition, TFBS data, and nucleosome occupancy of TFBSs are used as binding evidences.
These regulatory interactions are determined according to DNA binding evidences which are obtained from wet-lab experiments, such as site-directed mutation of transcription factor binding sites in its promoter region, ChIP, ChIP-on-chip, ChIP-seq and so on.
We apply our method on six TCGx datasets related to yeast cell cycle, and validate reliable GINs using the following three sources of experimental knowledge: biopathways from KEGG, protein-protein interaction networks and transcription factor-gene regulations with experimental DNA binding evidences.
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Additionally, the hydrogen bond pattern moieties of the unpaired nucleotides should be involved in Ag+ binding evidenced by a decrease of the redox signal when introducing a ligand with its hydrogen bond moiety complementary to the nucleotide deoxycytidine.
Based on the RegulomeDB database, we observed a minimal binding evidence of rs8050136, which has a strong LD with rs9939609.
We listed SNPs with regulatory potential and TF affinity differences as well as TAF binding evidence in Additional file 6.
The calculated regulatory potential, allelic binding affinity scores and ChIP-seq binding evidence were the three criteria used to prioritize candidates.
The first one was the TF binding network whose edges are supported only by TF binding evidence deposited in the YEASTRACT database [ 31].
Lastly, ChIP-seq data of transcription associated factors and topological domains were included as binding evidence and potential gene target inference.
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