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These results revealed that the proposed protein-imprinting technique using Hyp-En-Dans could provide a highly selective protein-sensing platform, in which only specific binding events would be detected by fluorescent measurements.
If CTCF and ER are interacting co-operatively, the cell-line unique CTCF binding events would be more likely to overlap with the cell-line unique ER binding events.
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The concentration independence at a high concentration of AlkA suggests that the εA quenching step being observed corresponds to the nucleotide flipping step (kflip,obs = 242 ± 11 s–1), as a DNA binding event would be dependent on AlkA concentration.
All three binding events would appear to compete with one another in the case of binding of MecI to the bla operator, so this scenario is distinct.
The vote was not expected to be compulsory and its result would be non-binding. .
If helix C was already displaced during transcription, the RNA binding process would be a single event.
Although these targets would not be internationally binding, they would be subject to outside verification.
The ballot is not binding, but defeat would be embarrassing for Corbyn.
For good reason too - an EU club without binding legal rules would be a mess.
But the team suggests that if the binding events can be made very short compared with the time between bindings, then simply recording a single impulse at each binding event and counting the number of impulses within a given period of time would be good enough.
The question has been raised therefore whether a large proportion of binding events are "opportunistic" rather than "functional" (John et al, 2011; Zhu et al, 2012), where "functional" would refer to those binding events that are relevant in terms of transcriptional control processes.
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