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The dynamic binding events with the formation of 1 1(L/M) and 1 2(L/M) complexes were examined.
We compared the number of registered binding events with the expected number from the continuous model.
We assessed this by overlapping the cell-line unique CTCF binding events with the cell-line unique ER binding events.
There are two ways to deal with this problem – either to take the information as is and to hope that machine learning procedures filter the aspects of the data that are relevant for prediction, or to formulate a physically reasonable model of productive binding events with the kinase directly.
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However, the fluctuation at this timescale does not tell us much, because the expected number of binding events with this rate at this timescale is 0.027.
Conversely, dynamic binding events with no change in the gene's transcript level can be viewed as non-functional.
In addition, around 70% of the binary-only DPs are the weaker binding events with log2 signals <2.
We attempted to correlate the HSF binding events with changes in gene transcription using standard expression microarray analysis of heat shocked Kc cells and Drosophila 3rd instar larvae.
However, we obtained a dramatic decrease on the percentage of binding events with erythrocyte aging.
This observation supports the hypothesis that, at least for a fraction of the footprints that contain no known protein-binding motif, the footprint is maintained in part by the presence of proximal binding events with higher sequence specificity.
Wide-field techniques such as total internal reflection fluorescence microscopy (TIRFM) can also be used to study such interactions by immobilizing one biological molecule on the surface and observing binding events with other biological molecules in solution.
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CEO of Professional Science Editing for Scientists @ prosciediting.com