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It has recently been shown that unlike TF binding events with high TF occupancy levels (measured by ChIP signal), genomic regions with low TF occupancy levels are not responsible for patterned reporter gene expression in Drosophila (Fisher et al., 2012).
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This observation supports the hypothesis that, at least for a fraction of the footprints that contain no known protein-binding motif, the footprint is maintained in part by the presence of proximal binding events with higher sequence specificity.
Consequently, the composite LysM1 LysM3 binding site provides a single binding event with ultra-high (pM) affinity for chitin binding, the highest chitin-binding affinity described in nature, which is extremely competent to sequester chitin oligosaccharides.
However, we obtained a dramatic decrease on the percentage of binding events with erythrocyte aging.
We assessed this by overlapping the cell-line unique CTCF binding events with the cell-line unique ER binding events.
An independent approach to associate TF binding events with gene expression outputs is to map mathematically between the binding events and gene expression patterns collected over many conditions.
We compared the number of registered binding events with the expected number from the continuous model.
They do not necessarily correspond to individual molecular binding events with a single TCR.
Interestingly, for 6His‐tagged G375D, we only detected one binding event with a K D of 0.39 ± 0.10 μM, suggesting that the mutation leads to the loss of the low‐affinity binding site and affects the binding affinity of the high‐affinity binding site.
Guo Y, Papachristoudis G, Altshuler RC, Gerber GK, Jaakkola TS, Gifford DK, Mahony S. Discovering homotypic binding events at high spatial resolution.
Toby acts his parts about certain events with high speed.
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