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They do not necessarily correspond to individual molecular binding events with a single TCR.
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Enzymes have been successfully used for detection of analytes providing both recognition and amplification of the binding event with a detectable readout.
Interestingly, for 6His‐tagged G375D, we only detected one binding event with a K D of 0.39 ± 0.10 μM, suggesting that the mutation leads to the loss of the low‐affinity binding site and affects the binding affinity of the high‐affinity binding site.
Nimblegen genome coordinates were converted to Release 5.0 of the Drosophila genome and genes were defined as targets in which a binding event (with an FDR of 0%) occurred within up to 5 kbp of the gene structure (depending on the proximity of adjacent genes).
A matrix of binding events with 270,261 genomic regions as rows and overrepresented ChIP-seq data sets (K) from GSCA step as columns is generated.
We have also performed transcription analysis of heat shocked Kc167 cells and 3rd instar larvae in an attempt to correlate HSF binding events with induction of gene transcription.
There are two ways to deal with this problem – either to take the information as is and to hope that machine learning procedures filter the aspects of the data that are relevant for prediction, or to formulate a physically reasonable model of productive binding events with the kinase directly.
However, we obtained a dramatic decrease on the percentage of binding events with erythrocyte aging.
The dynamic binding events with the formation of 1 1(L/M) and 1 2(L/M) complexes were examined.
We attempted to correlate the HSF binding events with changes in gene transcription using standard expression microarray analysis of heat shocked Kc cells and Drosophila 3rd instar larvae.
The interaction of penetratin with the zwitterionic PC/DOPE bilayer produced, like for PC, two binding events with negative shifts followed by positive shifts for both polarizations (Table 1).
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