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Potential binding events were defined as A) one oligonucleotide having a ratio within the upper 0.001 quantile of the total ratio distribution or B) two neighbouring oligonucleotides within a window of 1 kb where one oligonucleotide has a ratio within the upper 0.01 quantile and the other one has a ratio within the upper 0.05 quantile of the total ratio distribution.
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A potential binding event is defined with respect to the estimated average fragment size of the sonicated genomic DNA (550 bp) in relation to the distance of oligonucleotides relative to the promoter regions of the examined TSSs (distances between oligonucleotides is 100 bp).
Therefore, a potential binding event is defined as at least three oligonucleotides that fulfil the following criteria: a centre oligonucleotide has a ChIP/IP ratio within the upper 0.01 quantile of the total ratio distribution and one upstream and one downstream neighbour each within a distance of max.
A cell-line specific CTCF binding event was defined as a peak identified in both replicates of that cell line and in neither replicate of the other cell lines.
A bead-binding event was defined as a bead that remained attached to the microtubule for at least 2 s after the laser trap has turned off.
Severe adverse events were defined as events requiring medical advice.
Two types of new events were defined.
Adverse events were defined as any of the following.
The events were defined as death for the OS analysis.
Events are defined as the number of event nights, so if the circus plays twice that's two events.
Correct decoding events are defined as follows.
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CEO of Professional Science Editing for Scientists @ prosciediting.com