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In glycan arrays, HA must be directly or indirectly labelled with a fluorescent tag to allow for binding events to be detected (Stevens et al. 2006).
Biosensors consist of a physicochemical detector and a biological component, enabling binding events to be transduced, thereby allowing detection of very small amounts of target biomolecules (keratinase).
Knowing the PWM for a TF allows standard equations for reversible binding events to be used to estimate the probability of the bound and unbound state at a given site in the CRM.
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However, the issue of specific versus non-specific binding to chromatin/DNA was extensively addressed in our previous Cell paper (Chen et al, 2014b) and the relevant results that provide strong evidence for the long-lived binding events to represent site specific interactions are listed below: 1) We deleted the Sox2 DNA binding domain and tracked its movement.
Motif analysis was performed on the cell-line unique CTCF binding events to determine if there were any differences in enriched motifs in the cell-line unique CTCF binding events.
Because only one cooperative effect can be described in the 'attributeList' element of an interaction, an interaction that has more than one effect on one or more other binding events has to be repeated for each of these effects.
Conserved CTCF-binding events are less sensitive than species-specific binding events to reduced levels of the CTCF protein.
While the significance of the β5-ERK2 binding event remains to be determined we have postulated that a hierarchy of integrin-ERK2 interactions exists within cancer cells with preferential binding of the kinase to the growth-promoting β6 subunit when it is expressed (Ahmed et al, 2002).
One consideration about comparing singletons to CRMs is that singleton TF binding events are likely to become CRMs as more factors are tested and more peaks are called (see Figure 1 source data 1E for a comparison of the stability singleton and CRM categories).
If CTCF and ER are interacting co-operatively, the cell-line unique CTCF binding events would be more likely to overlap with the cell-line unique ER binding events.
Thus, SU HW) shows preferences for LAD borders as well as for the +40 kb position within LADs, but these SU HW) binding events appear not to be linked.
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