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From these adhesion events, approximately 75% were of single rupture events and the remaining 25% were from double or multiple steps binding events, for the three studied erythrocytes populations.
The typical numbers of binding events for the other lectins generally ranged between 1200 2100 for each set of 1024 curves (Supplemental Fig. S1 H).
This analysis identified 826, 2,386, and 591 human-specific binding events for the NANOG, OCT4, and CTCF TFs, respectively (supplementary data sets S4 S6, Supplementary Material online).
Consequently, it is likely that stochastic binding events instead of consistent cell culture sub-populations explain most of the observed cell-type specific binding events for the low and isolated peaks.
In this work, we studied statistical characteristics of protein-DNA binding events for the eleven stem cell- related transcription factors bound in the genome of mouse embryonic stem cells E14 and detected by ChIP-seq assay [ 15].
We have developed an algorithm with the potential to simulate a very high number of binding events for the 15 amino acid peptide theoretical combinations (>3.3 × 10) to each substrate.
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Our detailed structural and thermodynamic characterization of an important binding event for the coupling between replication and transcription (20) may aid the understanding of this process in papillomaviruses and in double-stranded DNA viruses in general.
Although the difference in binding events for Hth in the leg and haltere is less than 2-fold (∼1000 and ∼600, respectively), there are ∼5-fold more binding events for Ubx in the haltere compared to the leg (∼4600 and ∼900, respectively).
Related to Figure S1 To generate the heatmaps of the raw ChIP-seq data, the human binding events for CTCF that are within the multispecies alignment and have syntenic sequence on the human chromosome 21 in the Tc1 mouse were used as targets to center each window.
An example of binding events for each step of the protein expression profiling assay is provided in Figure S3.
Therefore, our strategies are not guaranteed to identify all the TFBS candidates, especially binding events for a single gene, because the system is designed based on co-expressed gene clusters.
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