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This second binding event could occur via free ends of the dT70, i.e., a few nucleotides at either end that remained unbound to the first tetramer.
Thus false oestrus events could occur at any point during the 22-month gestation.
However, we acknowledge that thrombotic events could occur a long time after PCC infusion.
To further extend these findings, we tested whether similar events could occur in ex vivo conditions.
A major event could occur within our lifetimes.
Some held that if the event could occur otherwise God would be fallible.
Although energetically coupling between the two binding events could not be resolved (analysis not shown), it is likely that it does exist because the second binding event is 1 order of magnitude tighter than nonspecific binding.
The effects of nucleotide and lipid A/daunorubicin binding to MsbA were additive, and binding could occur in any order.
Electrochemical experiments, however, indicated that anion binding could occur in the oxidised ferrocenium form as a consequence of electrostatic interactions.
For the catalytic cycle of DNA MTases, binding of substrates could occur in a random or sequential order.
They both rely on events that could occur very early in the oogenetic process.
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