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These binding events are also characterized by free energies of binding, Δ GRNA and Δ GU2A′, respectively.
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Detection of single biomolecular binding events was also demonstrated using nanoparticles as labels.
Multiple binding events were also confirmed with the software package SEDPHAT (Houtman et al., 2007 ).
ER/FOXA1/CTCF binding events were also significantly biased towards estrogen-regulated genes, compared to ER/FOXA1 binding events that do not have an overlapping CTCF binding event.
The same binding event is also observed in the T3 leg, although with apparently weaker affinity (Figure 4D).
This putative initial binding event is also apparent in the equilibrium-binding isotherm yielding an apparent Kd of ∼1.7 μM.
If the repeat-driven mechanisms currently active in creating functional CTCF-binding events were also active in the common mammalian ancestor, then ancestral expansions would eventually become shared binding events in descendant species, such as our study species.
These binding events are more likely to be associated with cell-line specific ER binding events and are also more likely to be adjacent to genes that are expressed in that particular cell line.
These two binding events are thought to not only delay the Tat transport event, but also actively facilitate efficient insertion of the MGD cofactor into the apoenzyme.
Many protein binding events are random and inconsequential.
Proximal binding events are enriched at genes with housekeeping functions, while distal binding events interact with genes involved in dynamic biological processes including response to stimulus.
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