While there is extensive coverage of the sequence examined, AC cleavage patterns for the two PAs show discrete binding events and do not overlap significantly.
As NMR has limited sensitivity, strong binding events and nonunitary stoichiometries are not always identified.
Further, the number of distinct Pho7 binding events varies between different promoters, and we do not observe a clear correlation between the number of binding events and transcriptional up-regulation.
This resulted in 7, 314 MCF-7-specific CTCF binding events, 2, 730 ZR75-1-specific CTCF binding events, and 1, 037 MCF10A-specific CTCF binding events.
Therefore, slow (relaxation time > \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\upmu $$\end{document} μ s) enzyme-biomass dissociation processes and similarly long binding events are not captured here.
This ambiguity makes it difficult to search for and identify all instances of an event subtype, e.g. the names used for binding events do not all contain the word bind, a text search for 'phosphorylates' does not identify all phosphorylation reactions.
Surface acoustic wave (SAW) biosensors allow the user to detect and quantify binding events, giving information not only on affinity (KD) and kinetics (kon and koff), but also on viscoelastic effects.
Unlike interactions of wt Httex1 with mHttex1monomers, wt-wt Httex1 monomer binding events do not result in IBs formation [74].
Additionally, there is no consistent arrangement of the Adr1 and Cat8 binding sites within promoters of co-regulated genes [9], supporting the conclusion that two separate activator binding events are not necessary for activation of these genes.
