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It is this preferential binding event that is responsible for enhanced MMP-9 secretion in cells when they express αvβ6.
On the basis of these results, we conclude that the third binding event that is observed in the isotherm corresponds to the interaction of an additional UP1 molecule with the unfolded G-quadruplex.
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Given that the authors acknowledge that this protein-protein interaction has previously been reported there would be some merit in further emphasizing the new aspects of the binding event that are demonstrated in this new data.
Taken together, it appears that E2F4 has a substantial number of binding events that are entirely dispensable for proper transcription of downstream genes, despite the well-characterized role it plays in cell cycle control.
As such, the unknown footprints may correspond to non-specific binding events that are facilitated by an adjacent specific binding event.
The assessment of correlation between transcriptional changes of the FFRP and its target genes revealed the subset of binding events that were conditionally functional in specific environmental contexts.
In the third and last set of RW simulations, we studied binding events that were registered during a transient response in the cleft.
Once it is possible to distinguish true binding events that are functional from those that are non-functional, we will also be able to investigate the molecular factors distinguishing one from the other.
The SRP concentration at which the smFRET experiments are performed is relevant only in that, at higher SRP concentrations, arrival times are faster such that the number of individual binding events that are observed is larger.
This overlap is higher than the number of CTCF binding events that were common to only one of the ER-positive cell lines and the ER-negative cell line (795 CTCF binding regions shared between MCF-7 and MCF10A and 1, 354 CTCF binding events shared between ZR75-1 and MCF10A), suggesting a link between CTCF and ER binding.
The question has been raised therefore whether a large proportion of binding events are "opportunistic" rather than "functional" (John et al, 2011; Zhu et al, 2012), where "functional" would refer to those binding events that are relevant in terms of transcriptional control processes.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com