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"Basically, any approach that really depends on having a single specific binding event may be compromised," he says.
Biophysical studies on Pho85, a kinase in S. cerevisiae homologous to Cdc28, further showed semi-processive phosphorylation i.e., one kinase-substrate binding event may be followed by several phosphate transfer events without dissociation of the enzyme and substrate proteins [37].
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The combined effect of multiple protein binding events may be the basis for effective competition with nucleosomes, consistent with the collaborative competition mechanism proposed by Miller and Widom [ 35].
One conclusion often drawn from this disparity is that a large proportion of TF binding events may be "opportunistic" rather than "functional," where it is thought that TFs bind to regions that happen to be accessible and contain relevant motifs, without this binding being particularly relevant in terms of transcriptional control processes.
A detected binding event may not be biologically relevant, or a known regulatory interaction might not be observed under the growth conditions tested so far.
However, in more complex cases as observed in Figure 3, a binding isotherm consisting of multiple events may be simplified into an isotherm that is an approximate average of all events as shown in Figure 5.
Now events may be forcing a rethink.
In any event, LILRA1 follows this rule: if it displays self-interaction of Lys42 with Glu68, the binding affinity may be reduced relative to LILRB1 or B2.
Phosphotyrosine ligand binding may be a key event in modulating PK activity regulated by the allosteric activator FBP [ 150].
Therefore, an early event following integrin β1-binding may be the induction of a specific pathway or pathways, which relate to Rho.
Our previous findings indicated that Ctcf (CCCTC-binding factor) may be an important player in key events whereby PARylation controls the unmethylated status of some CpG-rich regions.
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