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Hence, a specific (i.e., gene-specific) binding event does not induce formation of a single conformation, which corresponds to a given functional outcome.
[10, 11] Even if a binding event does not significantly change the size or charge of a protein, MST can still detect the binding owing to binding- induced changes in the molecules' solvation entropy.
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This ambiguity makes it difficult to search for and identify all instances of an event subtype, e.g. the names used for binding events do not all contain the word bind, a text search for 'phosphorylates' does not identify all phosphorylation reactions.
Unlike interactions of wt Httex1 with mHttex1monomers, wt-wt Httex1 monomer binding events do not result in IBs formation [74].
This choice was motivated by the assumption that the relevant binding events do not yet occur in the Thp samples [ 17].
The fact that many of the clustered binding events do not return to the zero-force baseline suggests that the lectin does not fully detach from the mucin chain but slides along it during the measurement phase as the PEG tether anchoring the lectin to the AFM tip clearly remains extended.
Also, since many of the ER functional binding events do not directly involve promoter regions, one can speculate that some DNA loops might circum vent the need for a transcription factor to be present at the promoter by concentrating the transcription machinery in the three-dimensional hubs.
Because protein-DNA binding events do not give information on the direction of the regulatory influence of the TF with respect to the transcription rate of its target genes, we complemented the results of the OCT4 ChIP-on-chip experiment with the results of the RNAi mediated OCT4 silencing in hESCs (H1 clone) performed by Babaie et al. [ 16].
The number of undetected genes was higher after amplification using the ADP system compared to unamplified RNA, furthermore the number of predicted ADP primer-binding events did not appear to be a significant factor in the loss of detectable genes from the ADP amplified analysis.
Importantly, this ubiquitination event does not aect Smad4 stability but inhibits its binding to phosphorylated Smad2.
The only universal consequence of a legally binding promise is, that the law makes the promisor pay damages if the promised event does not come to pass.
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