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In this case, interference can take place already, at the stage of initial binding, especially for B- and H-epitopes, but less prominently for A- and SiaLe-epitopes.
Steady-state kinetic experiments suggested that GNA synthesis by Therminator was affected by both decreased catalytic rates and weakened substrate binding, especially for pyrimidines.
Together, these results support that phosphorylated CBM binds the KD more potently than non-phosphorylated CBM and they demonstrate that binding of carbohydrates to the CBM strongly reduces CBM-KD binding, especially for the weaker interaction with non-phosphorylated CBM.
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Electrochemical investigations on the AP-DNA-modified electrodes reveal that Ag+ preferentially associates to the unpaired nucleotides instead of the coexisted base pairs and shows sequence-dependant binding, especially stronger for purines than for pyrimidines.
From BLASTx results, a majority of molecular function ontology results (≥1000 contigs) were for binding, especially protein and nucleic acid binding, and for hydrolase, catalytic, transferase, oxidoreductase, structural molecule, and ion transporter activity (Figure 3A).
Position-specific enrichment of uncapped 5′-ends was seen upstream of motifs recognized by several RNA binding proteins especially for the binding site of PUF proteins.
Because high affinity IgGs can compete with IgMs for antigen binding, especially during a secondary infection, an IgM capture assay is preferably used.
Mutagenesis studies performed either on the human [ 14- 17] or ovine MT1 receptor [ 18] and studies using human chimeric GPR50/MT1 receptor constructs [ 19, 20] have shown several highly conserved residues in transmembrane helices that are critical for ligand binding especially those in transmembrane helix III (TMIII: S110, G258) (Fig. 3).
For III-30, the HYP→PRO substitutions caused a reduction in hD1D2 binding, especially with III-30a.
The distinctive α-helix motif between two β-strands of the RNA-binding region may have a significant effect on binding specificity, especially for NVAV.
Furthermore, static binding capacities, especially for smaller ligands comprised of fewer B-domains, were improved up to 87mghIgGG mL−1 using site-specific coupling chemistry, which is an improvement of more than 20% compared to commercially available materials.
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