Sentence examples for binding equilibrium was not from inspiring English sources
The preincubated mixtures were added to the coated ELISA plate for 15 min at RT, after incubation these mixtures were transferred to another Aβ1-42 -coated plate and treated identically, to confirm that the binding equilibrium was not disturbed.
The progressive nature of binding reflected the relative excess of ligand, where binding equilibrium was not achieved.
An even slower approach toward binding equilibrium was previously observed by ChIP [28].
This loss of binding sites is considerably faster at 37 C than at 4 C. On the other hand, once binding equilibrium is reached between nuclear bound and free triiodothyronine the level of binding does not change during the time in which a considerable loss of binding had occurred when nuclei were preincubated in the absence of hormone.
On the basis of the TAM equilibrium binding studies, it was not possible to determine whether, for the singly occupied enzyme, the high-affinity TAM binding site is situated on the subunit that is bound to PAPS.
On-line monitoring of the reactions revealed that equilibrium of peptide binding was not reached even after 3 h.
However the expression levels of recombinant VGSCs are still very low, so equilibrium ligand-binding assays are not useful to follow recombinant expression, e.g. in HEK-293 or CHO cells.
Equilibrium dialysis experiments have shown that GTP binding is not dependent on SAM binding, and that the position 6 oxo group and the amino group at position 2 are important for guanine recognition by MoaA.
During competition, the binding equilibrium of the scFv to its target peptide was not disturbed as the control plate showed 95% signal as compared to the assay plate.
