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Here, NMR signals of UP1 were selectively irradiated, and site-specific binding epitopes were detected via saturation transfer to SL3ESS3.
In patients with persistent allergic reaction, seven IgE and six IgG binding epitopes were detected on BLG (Järvinen et al. 2001).
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Cry9C (as either the intact protein or fragments that retained binding epitope) was detected in maize bread, muffins, and polenta at levels that were 13%, 5%, and 3%, respectively, of that found in whole grain (de Luis et al., 2009).
A higher level of LM11-binding epitopes was detected in 02-5B-318R spikes in comparison with the FHB susceptible wheat genotype confirming the higher degree of xylan arabinosylation of 2-5B-318R spikes in comparison with SaragollaS.
The reported human epitopes were detected by multiple methods, including chromium-release assay, proliferation assay, tetramer binding, intracellular cytokine staining, and IFN-γ ELISpot of CD4 T cells.
For the M2 protein, consensus epitopes were detected in the membrane and transmembrane regions, even though this is the Amantadine-binding site.
Three epitopes were detected on both proteins (Fig. 3B).
Surprisingly, no Lea epitopes were detected.
Some ricin A chain epitopes were detected in Golgi vesicles.
Almost all allergen-specific antibodies were mutated, and binding to both conformational and linear allergen epitopes was detected.
Although high relative amounts (compared to LM15) of the CCRC-M1 epitope were detected in our glycan array analysis, IF only revealed weak binding, even after pectate lyase pretreatment, suggesting that CCRC-M1 epitopes might be masked by other polymers than HG or are soluble and lost during antibody-incubation procedures.
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