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However, both HLA-B*35-Px and -Py molecules are capable of binding epitopes that do not fit the conventional PY motif (Los Alamos Database, http://www.hiv.lanl.gov/).hiv.lanl.gov/
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It remains elusive whether the H epitope/acetamido binding site is required for the Le binding strains that do not bind the H antigen.
In this assay, sCad11 is first captured with an extracellular cadherin11 epitope antibody and then detected with a separate biotinylated extracellular cadherin11 epitope antibody that does not interfere with epitope binding by the first antibody.
Targeted experimentation will identify B-cell epitope changes that would abolish AR3C binding as well as changes that do not have detrimental effects.
Similarly, the Le epitope binding site is missing in NV that does not bind the seLe antigen.
Antibody #2 recognizes an epitope in the N-terminal half of Dkk-1 that does not affect Dkk1 binding to LRP6.
Five peptides that did not harbor already known T-cell epitopes were suspected to be T-cell stimulatory as their sequences indicated potential HLA-DQ2.5 binding.
It is clear that the Tat signal peptide binding epitope does not include the conserved arginine pair and instead possesses significant hydrophobic character.
Different HLA alleles have different binding affinities for peptides (epitopes) that can fit in their HLA peptide binding site.
Rationally designed protein protein interaction inhibitors mimic interfacial binding epitopes, specifically residues that contribute significantly to binding.
This suggests that POM1 binds an epitope that competes with POM6 for binding to PrP.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com