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The binding epitope of the two antibodies was mapped to hemagglutinin 1 (Haminomino acid residues 111 120.
Both the Fab moiety as well as the receptor ectodomain moiety seem to act as rigid bodies, thus these differences show that a small reorientation of BMPR-IA within the binding epitope of the antibody protein is possible.
Due to the rather large size of the antigen BMPR-IA, the binding epitope of the Fab is quite flat maximizing the contact between the CDR loops and the antigen.
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The conserved binding epitope of this novel broadly neutralizing scFv may become key in the design and implementation of vaccines or RNA interference against H5N1 viruses.
Also, because 78% of microcystin in M. aeruginosa cells colocalizes with the thykaloid membrane (Young et al. 2005) and is, therefore, in close proximity to the phycobilisome complex, it is possible that microcystin directly or indirectly interferes with the IgE binding epitope of this complex.
As an example, Figure 5A shows that scFv 43M2 injected at 500 nM was still able to bind to the E7 sensor chip saturated with scFv 51, suggesting the binding epitopes of the two scFvs to be distinct.
We here use NMR techniques to analyze the binding epitopes of the anti-oligo/polysialic acid monoclonal antibodies (mAb) A2B5 and 12E3.
To generate a basis for further characterization of Jmjd6 expression, we mapped the binding epitopes of the antibodies AB-10562 and AB-11632.
Finally, competition binding experiments using flow cytometry were carried out to test whether the binding epitopes of the selected scFv-Fcs would overlap with that of MM2/57.
Another possibility is that the liver toxicity caused by erb-38 could be due to different clearance in the liver, the binding stability of the toxin moiety, or the difference in the binding epitopes of the two antibodies.
This is expected since on the biosensor with the ligand being immobilized, the binding epitopes of the ligand act independently, thus a dissociation of the receptor analyte is irrevocable.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com