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However, crystallization experiments with either the 27-mer region A or shorter 11-mer region D binding epitope identified in this work were unsuccessful.
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Fifteen epitopes identified in this study are novel.
Fine epitope mapping by analysis of binding sequences identified in phage display experiments suggested that a peptide ligand containing the amino acid motif QPDRS might constitute part or all of the F1-40 epitope.
In BALB/c mice, 10 and eight nonoverlapping T-cell epitopes were identified in CON6 and CON-S, whereas eight epitopes were identified in 92UG37_A and HXB2/BAL_B.
Epitopes were identified in a homologous and a heterologous way.
In addition to the above neutralizing epitopes identified by antibody binding assays, several immunodominant epitopes were identified by selection of escape mutants using neutralizing mAbs.
In addition, the 3D crystal structure of TtrD is presented at 1.35 Å resolution and a nine-residue binding epitope for TtrD is identified within the TtrA signal peptide close to the twin-arginine targeting motif.
In another recent study [44], 200 predicted high affinity binding peptides from MCMV were screened and 15 epitopes identified ( = 7.5% hit rate).
A competitive flow cytometry-based binding assay was performed to further validate the identified hCXCL1 binding epitopes in different ELR+ CXC chemokines.
Two major T-cell binding epitopes have been identified, which are positioned between amino acids 112 123 and 142 156 [ 22].
Similarly, mapping of conserved proteins against experimentally known IEDB epitopes identified six epitopes which had exact epitope match in four proteins including three from earlier predicted six PAPs.
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