Demonstrating a strong association between immune escape and avidity for target cells, this study suggests that viral replication success depends on a competition between host-cell binding, and engagement by neutralizing antibodies.
Intriguingly, the ATP binding and engagement mutants abolished the fraction of covalent ring species retained in the agarose plug during the chromosome entrapment assay.
Bacterial ATP-dependent proteases can bind substrates directly at sequence motifs in the substrate's amino acid chain, and in this case the binding and engagement steps are difficult to separate structurally and may well be two facets of the same biochemical event.
Our findings establish a preponderant contribution to ligand binding from engagement of a CR domain that contains a DxDxD or DxDDD motif with a ligand that has a proximal pair of positive charges, though these charges may be contributed by lysine, arginine or even protonated histidine side chains.
To test what steps of the ATP hydrolysis cycle in Smc ScpAB are involved in the entrapment of chromosomal DNA, we made use of smc alleles harboring mutations that specifically prevent ATP binding (K37I), engagement of Smc head domains (S1090R) or ATP hydrolysis (E1118Q) (Hirano and Hirano, 2004).
Loading of DNA requires ATP binding and head engagement, with the possibility that DNA is loaded through the dimerization hinge, which may open on head engagement.
The important elements of scrummaging - engagement, binding of the front rows and feeding of the ball - have been truncated into a split second.
However, it severely compromised ATP binding and, consequently, engagement of the N-terminal domains; both essential activities for proper DNA mismatch repair.
Rho GTPases undergo a conformational change dominated by two mobile switch regions upon binding GTP, allowing engagement of downstream effectors.
The special tailoring of the Ar 4-HB-CoA TE aryl group-binding site for optimal 4-HB-CoA binding includes the engagement of the ring C(4 OH in an extensive hydrogen-bond network (see Figure 9B).
