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Contrary to this, experimental evidence suggests that the binding energy must be relatively high and virtually irreversible.
For these disorder-based interactions, the disordered partner "morphs" from disorder-to-order, and, therefore, some of the binding energy must be spent to overcome the higher entropy of the unfolded state.
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This energy must be paid by the binding energy between the enzyme and the DNA.
Consequently, a substantial amount of interaction energy must be involved in substrate binding too in order to lower the energy barrier sufficiently for transport to occur.
That energy must be replenished.
It takes energy to produce energy: pumps must be powered and vast quantities of seawater desalinated.
Simply put, energy in must be greater than energy out.
"Rules must be binding, violations must be punished, words must mean something".
It must be binding and must be able to be verified and enforced.
These binding forces must be broken to remove a contaminant.
Therefore, the binding must be mediated through other microtubule-binding protein(s), including motor proteins that provide energy for the movement.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com