Sentence examples for binding elements was used from inspiring English sources
For the reporter vectors, the pUC19 containing the firefly luciferase (LUC) reporter gene driven by the minimal TATA box of the 35S promoter plus five GAL4 binding elements was used for self-activation test.
A luciferase reporter construct that was under the transcriptional control of 6× SMAD binding elements was used to demonstrate BMP induced intracellular signalling cascade [ 44].
The firefly luciferase gene (LUC) driven by a mini-35S (TATA box) promoter with five copies of the GAL4 binding element was used as a reporter, and the Renilla luciferase gene driven by the Arabidopsis Ubiquitin3 promoter was used as an internal control.
As the RXRα homodimer binds to both response elements, hRXRα binding to each response element was used as a positive control.
Here, two epitopes/hot spots in close proximity (residing on different secondary structure elements) are used to generate binding affinity and specificity.
For each peak of Tup1 binding, the highest-scored array element was used for all further analysis.
The RD29 cis-element was used as a negative control and it did not show binding with a recombinant protein.
To test whether the MIA-TF method can be used to detect the activity of transcription factors in the nuclear extracts of cells, the NF-κB probe (NBS: dsDNA fragment with biotin-labeled and containing NF-κB binding elements) was first used to detect the NF-κB activity in the nuclear extracts of cells.
To assess if the polymorphisms present in the 3'UTR of ELOVL6 affect the disruption or creation of microRNA binding sites, a computer-assisted identification of potential microRNA binding elements was performed using the finder tool of patrocles programme [ 41].
The transcription factor binding elements were predicted using the known DNA-binding profiles (JASPAR, http://jaspar.genereg.net/) at the position of polymorphic sites (Primary profile similarity is up to 80%).
