Sentence examples for binding element was mutated from inspiring English sources

The potential ERR binding element was mutated from the Luc-2036 sequence (Fig. 2D, left panel), which has been demonstrated to inactivate this element previously [50].

The barrier function correlated with the binding of C/EBP and CTCF to the wild type CAAT element and variegated expression across generations when the CAAT element was mutated.

To verify that inhibition of reporter gene activity was dependent on a functional miR-134-binding site in the 3′UTR, we cloned a luciferase reporter where the critical seed region in the miR-134 responsive element was mutated.

As expected, when the E-box element was mutated, Ngn1 activation of the miR-9-2 promoter was abolished.

In MAB, both elements were mutated.

As expected, we observed a strong reduction of luciferase expression when the SMAD3 or FOXL2 elements were mutated or when the whole composite element was removed.

On the contrary, we observed bimodality (Figure 4D) when the SigA binding site was mutated.

When the 124/506B binding site was mutated, miR-124 still upregulated lucifease expression to 240% (p = 0.0030).

When the 148/152A binding site was mutated, miR-148 downregulated reporter gene expression to 47% (p = 0.0049).

When the 124/506A binding site was mutated, miR-124 no longer affected expression of the reporter gene.

When the binding site was mutated, no effect was observed compared with control vector.