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Luciferase expression was driven by the p53 DNA binding element of the human p21 gene and the resulting luminescence signal was normalized to the co-transfected Renilla plasmid.
Primers for the SMAD binding element of the SERPINE1 promoter were: F: 5′-GGGAGTCAGCCGTGTATCAT-3′ and R: 5′-TAGGTTTTGTCTGTCTAGGACTTGG-3′ [ 27].
We further studied the effect of these treatments on luciferase reporter activity driven by Sp1 binding element of the mTERT promoter [ 53, 54].
It is the DNA binding element of the core binding factor (CBF) transcription complex and is required for hematopoiesis as shown in knock-out mouse studies [ 75].
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The UK has so far opted out of all binding elements of the law.
Our objective was to develop small peptides based on essential binding elements of the natural protein inhibitor, Tendamistat.
An opinion is a formal document summarizing the binding elements of the agreed development plan.
However, the emergence of high-throughput screening facilities provides the resources to potentially identify key binding elements of the R-spondin proteins.
Selective binding might be determined by the architecture (transcription factor binding elements) of the DNA sequence but, importantly, RIP140 together with ERα regulates the transcription of mitogenic signals emanating from the mammary gland.
Because the Sp1 binding elements of the HIV-1 promoter have seven G runs, to gain information on their individual roles in a G-quadruplex formation, we analyzed several DNA oligonucleotides covering different sets of four G runs.
If the stable complex forms, LexA-Cdk7 binds to the DNA-binding element of the reporter genes HIS3 and lacZ and the VP19 domain recruits the transcription factors.
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