While the presence of a binding element does not confer regulation, MSI1 regulation of lipid metabolism may be more complex than simple direct regulation of SCD.
The consensus GATA3 binding element is not present in the 5′-promoter region of human PTH, suggesting that the observed enhancing effect of GATA3 is caused by an indirect pathway, rather than by the direct binding of GATA3.
The Ac-DAF-16 DBD bound to amplicons containing this element, but not individual oligos, suggesting that the new element requires surrounding sequence for DBD binding.
From these data we reasoned that although individual examples for composite CTCT/TR binding elements are not a general feature of CTCF binding regions, a number of functional TRE motifs in the vicinity of CTCF binding sites might exist.
Other AtNHX1-influenced transcripts encoding putative DNA binding elements have not been associated with abiotic stress response previously.
However, T-box and Smad binding elements do not form part of the same motif, and although they appear in close proximity to each other they have inconsistent spacing, orientation and strand orientation relative to each other.
Motif searches did not identify any classic binding elements, indicating that not all TR binding sites conform to variations of the classic form.
FOG-1 has a CPEB-like RNA-binding domain (Luitjens et al. 2000; Jin et al. 2001a; Merkel et al. 2013; Figure 5A), although its RNA-binding element is not yet known.
Cyclin A binding prevents PRMT1 binding but not PRMT5.
Upon deletion of MSN2 and MSN4, elevated expression of its target genes CTT1, GSY1/2 and ATG8 (all of which have Msn2/4 binding sites in their promoter elements), but not CIT2 (target of Rtg1/3), was reduced in hsf1-R206S, F256S cells (see Figure 5A).
