We found that the ratio of bead volume to liquid volume during the binding step was critical for the binding efficiency and has to be kept low, e.g. it should not be higher than 1 15, even if the plate is shaken with high speed (1500 rpm).
For example, mutations that alter transcription factor binding sites such that binding efficiency is reduced are hypothesized to lead to the down regulation of gene transcription, but the environments in which such down regulation will improve fitness are unknown.
Au and Jan conclude that their mutations alter 80S binding efficiency and that "ribosome positioning" is affected by these mutations, although the position of the toeprint does not change in their experiments.
For each PPRE data on its reported consensus, isoform specificity, in vivo and in vitro binding efficiencies and Pubmed IDs were collected (additional file 1).
With current trends in designing drugs to bind efficiently to multiple targets, a revision of the original binding efficiency definition is carried out.
On the contrary, the CX3CR1 V249T280 haplotype, characterized by high expression and binding efficiency, is associated with high risk of CAD and could be related to plaque vulnerability and rupture.
With respect to binding efficiency, this is dependent on the affinity of the probe to the target nucleic acid.
XBP-1-p overexpression improves binding efficiency which is further increased by ER stress thus supporting the idea of dose-dependent upregulation of VEGFA transcription under ER stress.
The PCR yield is the product of three efficiencies: (i) the annealing efficiency is the fraction of templates that form binary complexes with primers during annealing, (ii) the polymerase binding efficiency is the fraction of binary complexes that bind to polymerase to form ternary complexes and (iii) the elongation efficiency is the fraction of ternary complexes that extend fully.
The physicochemical characterization (particle size, polydispersity index, degree of RNA entrapment and RNA binding efficiency) has been evaluated as a function of the nature of the RNA derivative (acid form, diethylaminoethanol salt or core form) from torula yeast.
