Cell-clock dye is a redox dye, which is readily taken up by live cells.
Our SSB biosensor has significant advantages over other fluorometric helicase tracking systems, such as dsDNA binding dyes which may inhibit the reactions being studied.
The ELISA binding assay for 89Zr-bevacizumab gave a binding of 75%, which is optimal for this assay, and did not alter upon coupling of 1 of 2 eq of dye.
The percentage of viable cells was detected using the viability kit, consisting of two DNA-binding fluorescent dyes: SYBR-14 (which is membrane-permeant and thus stains all sperm with bright green fluorescence in the nucleus) and propidium iodide (PI; that only penetrates sperm nuclei with compromised membrane integrity, fluorescing red and overwhelming the SYBR-14 signal) [61].
Traditionally, metaphase chromosomes are stained with a DNA-binding dye, such as Giemsa stain, which is taken up readily by gene-poor A,T-rich genomic regions and results in a chromosome-specific banding pattern that can be used to differentiate chromosomes and identify abnormalities.
"The dyes are a problem, which is why we need innovation in dyes," he says.
By using propidium monoazide, which is a photoreactive DNA-binding dye, the presence of non-negligible amounts of amplifiable DNA at the spore surface was highlighted.
For the assay described herein, detection is based on the binding of the SYBR-Green I dye into double stranded PCR products, which is a sequence independent process.
To determine whether these mutants had microscopic cuticle lesions that were not obvious under dissecting microscope, we stained animals with the nuclear-binding fluorescence dye Hoechst 33258, which was previously used as a sensitive detection for cuticle integrity (Moribe et al. 2004; Thein et al. 2009).
