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After incubation, Sytox green (10 µM, Molecular Probes), a cell impermeant DNA binding dye, was added to the cells to detect extracellular DNA.
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Hoechst 33342 (Sigma-Aldrich), a DNA-binding dye, was added at a final concentration of 5 µg/mL, and the suspension was incubated in dark at 37°C for 1.5 h with interval mixing.
An experiment was conducted: emerald dye was added to the Doubs at Pontarlier.
(2.2) A fluorescent dye was added to Surfactant suspensions.
One μl of fluorescent dye was added to the suspension.
Hoechst dye was added to the cells for 5 min.
CF750 dye was added to the antibody solution at a 12 : 1 molar ratio of dye to antibody.
After 2 h, the DHR123 dye was added and cells were incubated for an additional hour.
After washing, anti-human immunoglobulin G (IgG) conjugated to Alexa Fluor 647 dye was added.
Hoechst 33342 dye was added at 5 ng/ml for 10 min.
Bromophenol blue (3 μl) as a loading dye was added to each reaction mixture after incubation.
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