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Putative signal peptides, transmembrane helices and nucleic acid binding domains were predicted using SignalP [53], TMHMM [54] and Helix-Turn-Helix [55], respectively.
Putative signal peptides, transmembrane helices and nucleic acid binding domains were predicted using SignalP [ 59], TMHMM [ 60] and Helix-Turn-Helix [ 61], respectively.
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Receptor-binding domains were predicted by analyzing hydrophobicity distribution on protein sequences.
** Genes containing one of two double-stranded RNA-binding domains were predicted to be Dicer-encoding genes.
In fish genomes, there is a general propensity to encode a higher number of proteins that, by virtue of conserved active amino acid motifs and retention of chitin-binding domains, are predicted to encode active chitinases.
The TM domains were predicted with TMHMM.
Additionally, MKRN1 contains four C3H zinc finger RNA-binding domains 19 and consistent with these domains is predicted to function as a ribonucleoprotein 19262727.
Amino acid changes in zinc finger domains or KRAB domains are predicted to affect DNA binding of the ZFP57 protein and its possible function, respectively.
Both these domains are predicted to adopt all-β fold with a jellyroll topology similar to the discoidin-type sugar binding domains.
The preliminary SAR had been discussed and the unique binding site in SH2 domain was predicted by molecular docking.
The modular architecture of SdaI, wherein one domain mediates DNA binding while the other domain is predicted to catalyze hydrolysis, distinguishes SdaI from previously characterized restriction enzymes interacting with symmetric recognition sequences.
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