Sentence examples for binding domains were obtained from inspiring English sources

Both collagen binding domains were obtained as fusion proteins from an Escherichia coli expression system and fluorescently labeled using either amine-reactive succinimide (CNA35) or cysteine-reactive maleimide (GST-α1I) dyes.

Sequence specific DNA binding domains were obtained from the DBD [ 38] resource.

The information about TF binding domains was obtained from the Pfam (Bateman et al., 2004).

The 5' region coding for the DNA binding domain was obtained by RT-PCR using 72 hpf cDNA as a template (forward primer 5'-TCA CTG ATG GAT CTG ATG GC-3' and reverse primer 5'-CTG AAT CTG ACG AAG TCG GA-3').

Ligand binding domain sequences were obtained from the PFAM database (PFAM code: PF00104), and given their higher variability when compared to DBDs, were filtered using 50% alignment coverage, but the same values for minimum (15%) and maximum (80%) identity.

Multifunctional protein binding domains are domains which can recruit more than one binding partner.

The binding mechanism, binding constants, binding sites and binding distance were obtained.

The early experiments were aimed at localizing the EHS laminin binding domains that were obtained after cleavage with proteolytic enzymes, especially elastase.

Protein structures of extracellular domain and DNA binding domain in EGFR protein were obtained from protein data bank (PDB) and visualized using UCSF Chimera visualization tool [ 33].

Domain functions were obtained from PFAM annotations.

Apicomplexan orthologs for the 45 P. falciparum predicted transcription factors are presented in Additional File 5. Known DNA-binding domains [ 3, 35] were obtained from version 23.0 of the Protein families database (Pfam) [ 36].