In this study, an artificial transcription activator containing the Cre1 binding domain linked to the Xyr1 effector and binding domains was designed and constitutively overexpressed in T. reesei RUT C30.
In addition to commonly used GFP-like proteins, unrelated types of FPs on the base of flavin-binding domains, bilirubin-binding domains or biliverdin-binding domains were designed.
For example, receptor tyrosine kinases (RTKs), transmembrane proteins consisting of an extracellular ligand binding site and an intracellular tyrosine kinase domain, are designed inactive until a ligand binds to the extracellular site of the RTK, which often induces RTK oligomerization [ 14].
Large scale changes in transcriptional activity are induced when the DNA binding domain is deliberately designed to have lower binding specificity.
In this study, protein-based nanoparticles composed of a hydrophobic elastin-like peptide unit, a hydrophilic aspartic acid-rich peptide unit and displaying antibody binding domains on their surfaces, were designed and genetically synthesized.
Artificial ZFP AZPP) technology allows DNA sequences to be selected directionally and a DNA-binding domain to be designed [ 10- 13].
Minimalist hybrids of the DNA-binding domain of bHLH/PAS protein Arnt and leucine zipper dimerization domain of bZIP protein C/EBP were designed to target the E-box.
The zinc-finger nucleases (ZFNs) are engineered endonucleases composed of a tandem array of zinc finger DNA-binding domains, which can be designed to selectively bind a DNA sequence of choice, coupled to the catalytic domain from the type IIS restriction enzyme FokI.
