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Most of the differences among the spike proteins in different betacoronavirus A1 members were also observed in the N terminal half of their spike protein, where the receptor binding domains should be located.
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Flexibility of the outer, myosin-binding domain should be functionally important, permitting attachment of myosin cross-bridges over a range of angles.
First, the antigen-binding domain should be unaltered, thereby maximizing the antibody function.
Within Apaf-1, the signal about the binding of cytochrome c to the WD domains should be mechanistically transmitted towards the nucleotide-binding domain.
As the Tiam2 PHCCEx domain binds acidic peptides, we supposed that a basic region on the PHCCEx domain should be responsible for peptide binding.
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Since DT385 does not possess a receptor-binding domain, it should be incapable of binding to cells and becoming internalized.
Their data demonstrated that on average 25% of breast cancers harbour missense mutations in either the kinase, helical or p85 binding domains, although it should be noted that only the three exons corresponding to these domains were sequenced in their analysis.
If the main purpose is to map a binding domain, small fragments should be used, while for identification of genes encoding binding proteins, larger fragments are to prefer.
Nevertheless, by taking advantage of methods such as high-density peptide microarrays and proteomic display methods, and focusing the efforts on less explored peptide binding domain families it should be feasible to largely expand the knowledge on the binding motifs in the proteomes within the next ten years.
In addition, the iron sulfur cluster binding domain of PscB should be water accessible, and PscD should be close to the chlorosomal side of the FMO trimer.
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