The rhesus rotavirus VP4 sialic acid binding domain has a galectin fold with a novel carbohydrate binding site.
The C-terminal cell wall binding domain has a stabilizing role in the recrystallization of CbsA monomers by allowing a more efficient sheet formation (Antikainen et al. 2002).
Therefore, a key question was whether hydrogen bonding between the phosphomonoester of PA and lysines and arginines in PA-binding domains had a role in binding specificity.
Mutagenesis studies focused on the reactive center loop, the hinge region, protease-binding exo-sites, conformational stability, and accessory ligand binding domains have led to a well-established serpin inhibitory mechanism and have defined specific biological interactions and functions for a number of serpins in development, homeostasis, and host defense.
To date, only the plant ATPase AtHMA2 has been functionally characterized after heterologous expression in yeast (ion selectivity, activity rate, direction of transport, catalytic phosphorylation, etc. [57]), and only its C-terminal metal binding domains have been expressed in a prokaryotic system.
The noncovalent binding domains have been suggested to enable a local increase in the protein/enzyme concentration in the cell wall.
The NAD+-binding domain has a core seven-strand β-sheet (with a 1-2-9-6-5-3-4 strand order) surrounded by five α-helices, one β-hairpin (β7 and β8), and two short 310-helices.
The etfA1 gene, encoding the electron-transfer flavoprotein alpha subunit, which contains an FAD-binding domain, has an A300T mutation.
The subunit tilting has occluded the original DNA binding site, but because the connector and mismatch-binding domains have moved, a large channel (∼35 Å wide) in MutS has become accessible, which could easily accommodate a DNA duplex (20 Å diameter).
Mutually exclusive binding of Ca2+-CaM and other proteins to a common binding domain have been shown to play a role in the regulation of NMDA (N-methyl-D-aspartic acid) receptors, TRP (Transient Receptor Potential) channels and metabotropic glutamate receptors.
