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A fusion protein of the BRK-DNA binding domain with a FLAG epitope tag was purified [16] and captured onto the anti-Flag antibody.
We have previously demonstrated that RKIP/Raf interaction is regulated by two mechanisms: phosphorylation of RKIP at Ser-153, and occupation of RKIP's conserved ligand binding domain with a phospholipid (2-dihexanoyl-sn-glycero-3-phosphoethanolamine; DHPE).
For bait and prey construction, the PCR products were cloned at EcoR1 sites of pGAD T7 (Pro, P10, P8, RdRp, VPg and CP) vector having the Gal 4 activation domain with a N terminal hemagglutinin (HA) epitope tag and pGBK T7 (MP and MP deletion mutants) vector having Gal 4 DNA binding domain with a N terminal c-Myc epitope tag.
We also found a most interesting fusion of anticodon binding domain with a serine-threonine kinase (PF14_0423) in P. falciparum.
The "RheoReceptor-1" is a fusion of the GAL4 DNA binding domain with a modified ecdysone receptor (EcR) ligand binding domain.
The variable domain of the HCAb (VHH) has a single binding domain with a specificity and affinity similar to conventional antibodies [ 4, 5].
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The largest family identified in P. patula (1 794 members) contained leucine rich repeat (LRR), toll/interleukin-1 receptor (TIR), nucleotide binding domain with an ARC motif (NB-ARC), golgi transport complex 5 (COG5) and poxvirus A32 protein motifs.
To define the position and number of CENP-A binding domains with a different approach, we designed a set of 27 primer pairs (Supplementary Table 1) spanning the 500 kb region.
Strahl and co-workers profiled several methyllysine binding domains with a peptide microarray containing 130 peptides with up to six simultaneous PTMs including lysine and arginine methylation, serine and threonine phosphorylation, and lysine acetylation.
As shown in Figure 6B, DOSS binds to the human PPARγ ligand-binding domain with a KD of 1,380 nM.
The C-terminal region, consisting of one histidine and eight cysteines, represented a zinc-binding domain with a potentially unconventional architecture.
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