Egr-1 regulates gene transcription by the specific binding of its DNA binding domain, which consists of three zinc fingers, to the consensus GC-rich regions in the promoter of its target genes [11].
Transcription factors can be made up of multiple protein domains, and these might include a DNA binding domain (which recognizes specific target DNA sequences) or a domain that interacts with the transcriptional machinery to regulate the expression of genes.
GRP78/BiP, a chaperone predominantly in the ER lumen, interacts with unfolded proteins through its C-terminal substrate-binding domain, which is tightly regulated by a conformational change that depends on ATP occupation of its NBD.
FOXG1 contains a highly conserved DNA-binding domain, which binds to specific DNA sequences and regulates gene expression (Yao et al, 2001).
Our results suggest that reversible phosphorylation may also regulate a non-catalytic function of PTEN, its ability to interact with regulatory proteins via its C-terminal PDZ-binding domain, which may control its localization, stability and activity [ 7].
Studies in C. elegans have also shown that Unc-51 interacts with Unc-14, a RUN domain protein, which regulates kinesin 1-dependent vesicle transport by binding to Unc-16/JIP3/JSAP1, a cargo adaptor for the kinesin motor proteins [22], [23].
(2) The second is the signaling domain, comprised of the aromatic rings, which regulates signaling pathways, including the protein phosphate 2/protein kinase C pathway, and (3) the hydrophobic domain, which is responsible for the binding of Vitamin E isoforms in circulating lipoproteins and biological membranes.
The LIM domain is a zinc-binding motif found in a growing number of eukaryotic proteins, which regulates gene expression, cytoarchitecture, cell adhesion, cell motility and signal transduction [ 14].
We first investigated the effects of the I424S mutation on formation of a stable UBA dimer, a feature of the wild-type UBA domain which negatively regulates ubiquitin-binding [27].
Here, two tRNA synthetase domains are linked by an RNA binding domain that regulates gene expression through controlling translation [37], [38].
