The spectrin superfamily of proteins can form complexes via their actin binding domains, which interact with a number of other proteins, such as RACK-1, protein 4.1 and dynactin [25], [55], [56].
First, we identified the amino acid residues, located on the KRS anticodon-binding domain, which interact with the C-terminal extension of the LR.
The second conserved region is the phosphotyrosine binding (PTB) domain, which interacts with NPXY motifs in activated receptors [ 22, 23].
In eukaryotic PIWI proteins, in order from the N-terminus, these include the so-called "N-term" domain implicated in unwinding of the double-stranded guide and passenger strands and also guide-target duplexes [ 34] and the single-stranded RNA-binding PAZ domain which interacts with 3′ ends of guide strands.
For HIF-1αB, the bHLH-PAS domain, which is responsible for heterodimerization with HIF-β and DNA binding, was conserved; and the ODD domain, which interacts with PHD, was mutated [ 10, 19, 22, 66, 67].
One of the differences between ey and toy has been attributed to the PST domain, which interacts with disparate binding partners13.
The CAGpolyQ tract in the AR protein lies in its N-terminal transactivation domain which interacts with the C-terminal ligand binding domain (the N/C interaction).
The mature complex is then established by the binding of a co-chaperone that bears a TPR (tetratricopeptide) repeat domain which interacts with Hsp90.
Transcription factors can be made up of multiple protein domains, and these might include a DNA binding domain (which recognizes specific target DNA sequences) or a domain that interacts with the transcriptional machinery to regulate the expression of genes.
Nectins interact with actin-binding protein afadin, an α-catenin interacting protein, through the C-terminal PDZ binding domain, which predicts an involvement with the cadherin/catenin adhesion complex (Giagtzoglou et al., 2009).
