The IR then undergoes a conformational change in its intracellular β subunits that exposes its ATP-binding domain, which enables ATP binding and autophosphorylation.
Family members share an architecture consisting of a conserved FAD-binding domain and a more variable substrate-binding domain, which enables varying interactions with a range of substrates while maintaining the same cofactor-binding fold.
Ent3p and epsinR contain an amino-terminal phosphoinositide-binding domain, which enables binding to lipids, and each contains a clathrin-binding motif (58).
HDAC6 contains two intact HDAC catalytic domains, which execute deacetylation of α-tubulin (Zhang et al, 2006; Zou et al, 2006; Boyault et al, 2007a), and a ubiquitin-binding zinc-finger domain, which enables binding to ubiquitinated proteins (Seigneurin-Berny et al, 2001; Hook et al, 2002; Boyault et al, 2006).
DUOX contains a calcium-binding EF hand domain, which enables calcium to regulate the synthesis of H2O2 [ 24] and is therefore a good candidate for an effector of the calcium flash.
In order to asses the affect the binding abilities of the different targeting moieties the fUSE5-ZZ, phage vector that polyvalently displays the ZZ domain which enables the phages to form a stable complex with target-specific antibodies was used.
A fourth domain which enabled reflection on the knowledge was found as well, the domain of execution.
Another protein that interacts with the Smads is high mobility group protein A2 (HMGa2), a non-histone chromatin binding factor containing three A/T hook domains, which enable it to bind to A/T-rich sequences in the minor groove of DNA [ 39].
Parvins contain an actin-binding domain comprising 2 tandem calponin homology domains which enable parvins to recruit actin filaments (F-actin) to FAs and associate to stress fibers.
Calmodulin dependent protein kinase I (299 320) binding domain, which is a putative CaM-binding region, was obtained from AnaSpec.
Residues 1 150 form the NADPH binding domain, which is a variant of the typical dinucleotide-binding fold.
