The ability of Tat to bind to the TAR element through its basic RNA binding domain is also important for viral replication, because loss of this region results in a significant reduction in HIV Tat transactivation [36], [39].
For RolR, the DNA binding domain is also distinct from that observed in other TetR members.
This blockade may suggest that the TNF-receptor binding domain is also necessary for activity, and that an additional APRIL-specific receptor might exist.
Furthermore, Rouault et al. also demonstrated that not only is the calcium binding loop region involved in binding to the receptor, but the interfacial binding domain is also involved.
The eIF4G binding domain is also the site of interaction of a range of eIF4E binding proteins that use the same YXXXXLφ sequence to competitively bind eIF4E, preventing eIF4E eIF4G interactions and inhibiting cap-dependent translation and/or targeting mRNAs to specific locations [ 34, 60].
Orthologs of two other C. elegans genes (H02I12.1 and W03F11.1) encoding proteins containing putative chitin binding domains, were also identified.
Putative binding domains were also identified for the motif "anaphase-promoting complex (APC/C) binding site through the destruction box" (residues 28 to 36), for cyclins (residues 161 to 165), for MAPK (residues 167 to 176) (Fig. 1A).
Moreover, two acetylation sites of IRF-1 protein (Lys-39 and Lys-78) within the DNA-binding domain were also revealed.
Homologous binding domains are also found in other Gram-positive bacteria [ 26, 27] but further studies would be needed to determine if re-attachment also occur on other bacteria and if this is a parameter for neutralisation with the attached construct.
In addition to the domain architectures involved in the characterized functional groups, a cyclic nucleotide-binding domain was also fused to various domains involved in pyridine nucleotide-disulfide oxidoreductase, acetyltransferase, thioredoxin reductase, glutaminase, rhodanese, ferredoxin, and diguanylate cyclase, implying the versatile functions of cyclic nucleotide-binding proteins.
