Generally, these are organized with a DNA-binding domain that acts as a positive regulator of transcription fused to an effector domain which provides a binding site for a specific chemical activator molecule [20], [21].
Both these receptors contain an evolutionally conserved cAMP-binding domain that acts as a molecular switch for sensing intracellular cAMP levels to control diverse biological functions [ 63].
BESS domain is a protein binding domain that can interact each other or with other domains.
Mutations in TRPC6 that decreased PIP3 binding suppressed channel activity, as did an Akt-PH domain that acted as a PIP3 sponge (Kwon et al. 2007).
All SR proteins have one or two N-terminal RNA-binding domains (RBDs; also known as RNA recognition motif [RRM]) that provide RNA-binding specificity, and a C-terminal domain rich in Arg-Ser dipeptides (RS domain) that act as nuclear localization signals and facilitate interaction between different splicing factors (Caceres et al, 1997; Hertel & Graveley, 2005; Manley & Krainer, 2010).
However, all fungi contain a novel soluble SNARE protein, Vam7, which carries an N-terminal PX-domain that acts as a phosphoinositide binding module.
Our crystal structure and targeted mutational analyses demonstrate that the DUF1115 domain acts as a ssRNA-binding domain that specifically recognizes the first 10 nts of the U6 3′-overhang.
As PH-20 has a HA-binding domain that is distinct from its hyaluronidase domain, the molecule may also act as a HA receptor at the cell surface.
ING1b contains several conserved domains that act as binding regions for chromatin marks such as trimethylated histone H3 lysine 4 (H3K4me3), and chromatin-modifying complexes.
The latter include an aspartic acid located in the WMNDPNG motif (also known as β-fructosidase motif or sucrose-binding box) of domain A that acts as a nucleophile, and an aspartic acid located in the RDP motif of domain D that acts as a transition-state stabiliser, as well as a glutamic acid located in the EC motif of domain E that acts as the acid/base catalyst [ 3].
