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It is highly conserved across species, with 100% amino acid conservation in the DNA binding domain of all mammalian HNF4α.
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Although the basic amino acid rich DNA-binding domain of all these factors is highly conserved, binding to ABRE was demonstrated only with OSBZ8 and TRAB1.
Detailed information about the ligand-binding sites in nAChRs, necessary both for understanding the receptor functioning and for rational drug design, became available when the X-ray structures were solved for the acetylcholine-binding proteins (AChBP), excellent models for the ligand-binding domains of all Cys-loop receptors.
Here, we utilized protein-binding microarrays (PBMs) to compare the binding site preferences of isolated CSL with the preferred binding sites of CSL when bound to the CSL-binding domains of all four different human Notch receptors.
The DNA-binding domains of all TetR proteins show sequence homology; however, their C-terminal domains do not.
To probe the specificity of FOG-3 interaction with diverse CPEBs, we coexpressed FOG-3::FLAG with the RNA-binding domains of all four C. elegans CPEBs.
Figure 2E shows the expression levels of GAL1-driven PATs, tagged at the C-terminus with the IgG binding domain of Protein A. The functionality of all these constructs was tested in complementation assays of the corresponding deletion mutants: Figure 1 for Pfa3, Figure 2 for Swf1, and Figure 3 for Pfa4.
The conserved EF-hand calcium binding domain of 174 amino acids is common to all caleosins.
Cargo binding domain of yeast M yosin V is an all-alpha fold containing 15 amphipathic α-helices connected by short and long loops organized into two five-helical bundles.
All of them have a typical binding domain of 52 residues spanning a single C2/C2 zinc finger structure (DOF domain).
For instance, H1 binds to the H1 helix of the DNA binding domain of MYC.
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