This mutant VP1 retains its DNA-binding domain (localized at the N-terminus) and full DNA-binding activity, while it cannot assemble into capsids [43].
A protein function is carried out by a specific domain localized at a specific position.
Targets for synthetic peptides were selected if proteins have known peptide-binding domains and localize at the cytoplasm and/or nucleus based on the notion that peptides can penetrate lipid bilayer.
For instance, many large proteins are built from multiple domains with ligand binding sites localized at the interdomain clefts.
A series of mutants of the MeCP2 DNA binding domain fail to localize to heterochromatin in fixed cells, but localize indistinguishably from wildtype protein when living cells are examined by fluorescence microscopy.
EBNA1 is a DNA-binding protein localized at cellular chromatin via its chromosome-binding domains [51].
The adhesive domain is localized at the edges and anchored to the ECM.
The Sp family proteins, containing the conserved DNA-binding domain, are localized primarily within subnuclear foci associated with the nuclear matrix.
Novel chimeric proteins combining potent angiogens with extracellular matrix binding domains may localize to exposed matrices and provide sustained activity to promote endothelial regeneration after vascular interventions.
These binding sites were mainly localized at enhancers and were enriched for ERα, FOXM1, CREB and ATF3 binding motifs.
All AKAPs contain an A kinase binding domain (AKB) and a unique targeting domain localizing the PKA-AKAP complex to defined subcellular structures, membranes or organelles.
